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| Status |
Public on Apr 15, 2008 |
| Title |
Microarray analysis of rice plants exposed to jasmonic acid (JA) |
| Platform organism |
Oryza sativa Japonica Group |
| Sample organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, integrated transcriptomics and proteomics approaches were applied to investigate the molecular responses of JA in the shoots of 7-days old rice (cv. Nipponbare) seedlings exposed to 5 micromolar JA for a period of 7 days. Based on the morphological alteration of JA-exposed rice seedlings, transcript profiling of rice genes was performed in seedlings using rice DNA microarray chip, and proteomics by 2-DGE. This systematic survey showed that JA triggers a chain reaction of altered gene and protein expressions involved in multiple cellular processes in rice growth and development and defense.
Keywords: JA exposure response
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| Overall design |
An in vivo 7-days-old rice seedling model system was used, where whole seedlings were used for exposure to the JA (5 micromolar). Dye-swap or reverse labeling with Cy3 and Cy5 dyes procedure was applied followed by hybridization and wash processes, and the hybridized microarrays (G4138A) were scanned using a GenePix microarray scanner followed by the Gene Pix 4000 analysis application program for image analysis and data extraction processes. The GeneSpring Ver. 4 software was used for normalization.
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| Contributor(s) |
Rakwal R |
| Citation(s) |
17711327, 18517257 |
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| Submission date |
Apr 13, 2008 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Randeep Rakwal |
| E-mail(s) |
rakwal-68@aist.go.jp
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| Fax |
+81 29 861 6066
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| Organization name |
AIST
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| Lab |
MST, HTRC lab
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| Street address |
AIST WEST, Onagawa 16-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8569 |
| Country |
Japan |
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| Platforms (1) |
| GPL892 |
Agilent-012106 Rice Oligo Microarray G4138A (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA106845 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11158_RAW.tar |
3.3 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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