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Sample GSM281560 Query DataSets for GSM281560
Status Public on Apr 15, 2008
Title JASMONIC ACID_Shoot2
Sample type RNA
 
Channel 1
Source name JA_Control_Replication1
Organism Oryza sativa
Characteristics Healthy plants, mock
Treatment protocol Healthy seedlings at 7 days, control
Growth protocol Plants were grown in MS medium in growth chamber for 7 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy5
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
Channel 2
Source name JA_Treated_Replication1
Organism Oryza sativa
Characteristics Treated plants
Treatment protocol Treated with JA for 7 days
Growth protocol Plants were grown in MS medium in growth chamber for 7 days
Extracted molecule total RNA
Extraction protocol TRIZOL Reagent method
Label Cy3
Label protocol Standard Protocol of Agilent Low RNA Input Fluorescent Linear Amplification Kit
 
 
Hybridization protocol Standard protocol of Gene Expression Hybridization Kit from Agilent Technologies.
Scan protocol Standard protocol of Gene Pix Scanner
Description Keyword = rice
Keyword = JA
Data processing The GeneSpring Ver. 4 software was used for normalization.
 
Submission date Apr 13, 2008
Last update date Apr 14, 2008
Contact name Randeep Rakwal
E-mail(s) rakwal-68@aist.go.jp
Fax +81 29 861 6066
Organization name AIST
Lab MST, HTRC lab
Street address AIST WEST, Onagawa 16-1
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8569
Country Japan
 
Platform ID GPL892
Series (1)
GSE11158 Microarray analysis of rice plants exposed to jasmonic acid (JA)

Data table header descriptions
ID_REF
VALUE Log10 ( Norm_CH2 / Norm_CH1)
Norm_CH1 Normalised Intensity of Raw_CH1
Norm_CH2 Normalised Intensity of Raw_CH2
RAW_CH1 Background subtracted Intensity of CH1
RAW_CH2 Background subtracted Intensity of CH2

Data table
ID_REF VALUE Norm_CH1 Norm_CH2 RAW_CH1 RAW_CH2
1 -0.584141558 641 167 629 169
2 -0.572983512 621 166 607 162
3 -0.860338007 29 4 29 2
4 -0.014977156 118 114 118 113
5 -0.039040904 221 202 220 204
6 -0.507084478 45 14 45 13
7 -0.289748985 76 39 78 34
8 -0.016867925 210 202 207 195
9 -0.711204461 36 7 35 3
10 -0.114473303 82 63 79 58
11 -0.250420002 89 50 90 49
12 -0.023241683 403 382 395 385
13 -0.091770373 126 102 127 103
14 0.007686829 280 285 279 284
15 -0.095506799 309 248 310 241
16 -0.035715552 38 35 39 34
17 -0.102859346 147 116 147 114
18 0.034936978 191 207 183 203
19 -0.150874471 92 65 92 62
20 -0.189626738 277 179 260 171

Total number of rows: 22575

Table truncated, full table size 710 Kbytes.




Supplementary file Size Download File type/resource
GSM281560.gpr.gz 1.4 Mb (ftp)(http) GPR
Processed data included within Sample table

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