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| Status |
Public on Mar 29, 2008 |
| Title |
Microarray analysis of rice plants irradiated with carbon ion beams (HIMAC) |
| Platform organism |
Oryza sativa Japonica Group |
| Sample organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
In this study, carbon-ion beams generated via the heavy-ion medical accelerator in Chiba (HIMAC) were targeted to growing rice seedlings (7-days-old) to examine their effect on this genome model. Both physiological parameters, such as growth, and molecular events, specially the gene expression profiles were examined immediately after irradiation (at 270 Gy dose) by rice oligo (22K) DNA microarrays. Genome-wide transcriptional profiling of radiation-response genes in rice provides us with first report on how our radioactive environment affects a plant species.
Keywords: Irradiation response
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| Overall design |
An in vivo 7-days-old rice seedling model system was used, where whole seedlings were used for exposure to the emitted radiation from HIMAC (carbon ion beams). Dye-swap or reverse labeling with Cy3 and Cy5 dyes procedure was applied followed by hybridization and wash processes, and the hybridized microarrays (G4138A) were scanned using a GenePix microarray scanner followed by the Gene Pix 4000 analysis application program for image analysis and data extraction processes. The GeneSpring Ver. 4 software was used for normalization.
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| Contributor(s) |
Rakwal R, Shibato J, Nojima K, Kim Y, Nahm B, Jwa N, Endo S, Tanaka K, Iwahashi H |
| Citation(s) |
18414000 |
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| Submission date |
Dec 04, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Randeep Rakwal |
| E-mail(s) |
rakwal-68@aist.go.jp
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| Fax |
+81 29 861 6066
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| Organization name |
AIST
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| Lab |
MST, HTRC lab
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| Street address |
AIST WEST, Onagawa 16-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8569 |
| Country |
Japan |
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| Platforms (1) |
| GPL892 |
Agilent-012106 Rice Oligo Microarray G4138A (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA103697 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9765_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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