|
Status |
Public on Oct 25, 2007 |
Title |
New Mock 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
TW16-1263 Mock 1
|
Organism |
Oryza sativa |
Characteristics |
leaves of 14-day old seedling
|
Treatment protocol |
7-day old leaves were inoculated with three green leafhoppers (GLH) for 24 hours. Leaves were harvested 7 days after inoculation.
|
Growth protocol |
Seedlings were grown in greenhouse set at 28C for 14 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
|
|
Channel 2 |
Source name |
TW16-1029 Mock 1
|
Organism |
Oryza sativa |
Characteristics |
leaves of 14-day old seedling
|
Treatment protocol |
7-day old leaves were inoculated with three green leafhoppers (GLH) for 24 hours. Leaves were harvested 7 days after inoculation.
|
Growth protocol |
Seedlings were grown in greenhouse set at 28C for 14 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
Label |
Cy5
|
Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
|
|
|
Hybridization protocol |
in-situ Hybridization Kit Plus (Agilent Technologies) according to the manufacturer's instrcution.
|
Scan protocol |
DNA microarray scanner (Agilent Technologies) according to the manufacturer's instrcution.
|
Description |
TW16-1263 and -1029 were sister NIL (BC6) derived from the cross between cultivars TN1 (recurrent parent) and Utri Merah (donor parent). TW16-1263 is susceptible to rice tungro spherical virus (RTSV), and TW16-1029 is resistant to RTSV.
|
Data processing |
Feature Extraction Vert. 8.1 (Agilent Technologies)
|
|
|
Submission date |
Oct 25, 2006 |
Last update date |
Nov 06, 2006 |
Contact name |
IL-RYONG CHOI |
E-mail(s) |
ichoi@cgiar.org
|
Organization name |
International Rice Research Institute
|
Street address |
DAPO Box 7777
|
City |
Metro Manila |
ZIP/Postal code |
0000 |
Country |
Philippines |
|
|
Platform ID |
GPL892 |
Series (1) |
GSE6125 |
Comparative gene expression analysis between virus-susceptible and -resistant rice lines (TW16-1263 and -1029) |
|