|
| Status |
Public on Oct 25, 2007 |
| Title |
New RTSV 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
TW16-1263 RTSV 2
|
| Organism |
Oryza sativa |
| Characteristics |
leaves of 14-day old seedling
|
| Treatment protocol |
Leaves of 7-day old seedlings were inoculated 3 green leafhoppers carrying rice tungro spherical virus for 24 hours. Leaves were harvested 7 days after inoculation.
|
| Growth protocol |
Seedlings were grown in greenhouse set at 28C for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
| |
|
| Channel 2 |
| Source name |
TW16-1029 RTSV 2
|
| Organism |
Oryza sativa |
| Characteristics |
leaves of 14-day old seedling
|
| Treatment protocol |
Leaves of 7-day old seedlings were inoculated 3 green leafhoppers carrying rice tungro spherical virus for 24 hours. Leaves were harvested 7 days after inoculation.
|
| Growth protocol |
Seedlings were grown in greenhouse set at 28C for 14 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol according to the manufaturer's instruction.
|
| Label |
Cy5
|
| Label protocol |
Agilent Low RNA input fluorescent linear amplification kit according to the manufacturer's instrcution.
|
| |
|
| |
| Hybridization protocol |
in-situ Hybridization Kit Plus (Agilent Technologies) according to the manufacturer's instrcution.
|
| Scan protocol |
DNA microarray scanner (Agilent Technologies) according to the manufacturer's instrcution.
|
| Description |
TW16-1263 and -1029 were sister NIL (BC6) derived from the cross between cultivars TN1 (recurrent parent) and Utri Merah (donor parent). TW16-1263 is susceptible to rice tungro spherical virus (RTSV), and TW16-1029 is resistant to RTSV.
|
| Data processing |
Feature Extraction Vert. 8.1 (Agilent Technologies)
|
| |
|
| Submission date |
Oct 25, 2006 |
| Last update date |
Nov 06, 2006 |
| Contact name |
IL-RYONG CHOI |
| E-mail(s) |
ichoi@cgiar.org
|
| Organization name |
International Rice Research Institute
|
| Street address |
DAPO Box 7777
|
| City |
Metro Manila |
| ZIP/Postal code |
0000 |
| Country |
Philippines |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE6125 |
Comparative gene expression analysis between virus-susceptible and -resistant rice lines (TW16-1263 and -1029) |
|
