|
| Status |
Public on Feb 09, 2017 |
| Title |
Liver_High-fat diet_rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver, high-fat diet, 16 weeks
|
| Organism |
Mus musculus |
| Characteristics |
tissue: liver
treatment: high-fat diet
strain: C57/BL6
gender: male
age: 8 to 10 week old
|
| Growth protocol |
Liver tissues were collected and frozen immediately in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed using mirVanaTM RNA Isolation Kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Total RNA were tailed with Poly A and then labeled with Biotin.
|
| |
|
| Hybridization protocol |
The labeled RNA were hybridized for 16 hr at 48°C on Affemetrix miRNA 3.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The arrays were scanned by the Affymetrix Scanner 3000 (Affymetrix).
|
| Description |
non-exercised mice remained sedenrary
|
| Data processing |
Affymetrix GeneChip Command Console software (version4.0, Affymetrix) was used to analyze array images to get raw data and then offered RMA normalization. Next, Genespring software (version 12.5; Agilent Technologies) was used to proceed the following data analysis. Probes that at least 75 percent of samples in any 1 condition out of 2 conditions have flags in P were chosen for further data analysis.
Differentially expressed miRNAs were then identified through fold change as well as P value calculated using t-test. The threshold set for up- and down-regulated genes was a fold change>= 2.0 and a P value<= 0.05.
|
| |
|
| Submission date |
Feb 17, 2015 |
| Last update date |
Feb 09, 2017 |
| Contact name |
Yihua Bei |
| E-mail(s) |
beiyh36@yahoo.fr
|
| Phone |
+86 13816265303
|
| Organization name |
Shanghai University
|
| Department |
School of Life Science
|
| Lab |
Regeneration Lab
|
| Street address |
133 Nanchen Road
|
| City |
Shanghai |
| ZIP/Postal code |
200444 |
| Country |
China |
| |
|
| Platform ID |
GPL16384 |
| Series (1) |
| GSE65978 |
microRNA data from high-fat diet fed mice with and without exercise |
|
