|
| Status |
Public on Mar 01, 2017 |
| Title |
Resistant-RG5-3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Universal Mouse Reference
|
| Organism |
Mus musculus |
| Characteristics |
sample type: reference
|
| Treatment protocol |
melanoma tumor biopsies
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Fibrous Tissue Mini following manufacturers protocol
|
| Label |
Cy3
|
| Label protocol |
25ng of total RNA was reversed transcribed using Agilent Low Input Quick-Amp Labeling Kit and labeled with Perkin Elmer Cy5
|
| |
|
| Channel 2 |
| Source name |
Sample: MouseTissue Biopsy_120 day treated
|
| Organism |
Mus musculus |
| Characteristics |
tissue: melanoma
|
| Treatment protocol |
melanoma tumor biopsies
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using RNeasy Fibrous Tissue Mini following manufacturers protocol
|
| Label |
Cy5
|
| Label protocol |
25ng of total RNA was reversed transcribed using Agilent Low Input Quick-Amp Labeling Kit and labeled with Perkin Elmer Cy5
|
| |
|
| |
| Hybridization protocol |
Fragmentation Buffer and Hybridization Buffer (Agilent Gene Expression Hybridization Kit) were added, and samples applied to Agilent SurePrint G3 Mouse GE 8x60K microarrays
|
| Scan protocol |
Scanned on Agilent scanner
|
| Description |
120 day treated
|
| Data processing |
Images were quantified using Feature Extraction Software
|
| |
|
| Submission date |
Mar 14, 2016 |
| Last update date |
Mar 01, 2017 |
| Contact name |
Tammara Wood |
| E-mail(s) |
tammara.a.wood@dartmouth.edu
|
| Organization name |
Dartmouth Medical School
|
| Department |
Biomedical Data Science
|
| Lab |
Whitfield Lab
|
| Street address |
WTRB 674
|
| City |
Lebanon |
| State/province |
NH |
| ZIP/Postal code |
03756 |
| Country |
USA |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE79206 |
Acquired resistance to BRAF-inhibitors restores melanoma tumor immune suppression |
|
