|
| Status |
Public on Aug 31, 2009 |
| Title |
RTSV-infected TW16-4, rep 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
RTSV TW16-4, rep 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Near isogenic line from Utri Merah and Taiching Native 1
Tissue: two youngest leaves
|
| Treatment protocol |
Growth stage: two leaves visible
Light condition: Natural conditions
Temperature: 28±3℃
Virus inoculation: rice tungro spherical virus
Virus inoculation: 3 viruliferous GLH (Nephotettix virescence) per 1 seedling
Virus inoculation: 24 hours
|
| Growth protocol |
Pre-treatment:
Growth environment: greenhouse
Growth: pot culture (D: 120mm x H: 150mm)
Growth media: Soil collected fielf of International Rice Research Institute, Philippines. Autoclaved prior to use.
No. of seedlings per pot: 5
Light condition: Natural conditions
Temperature: 28±3℃
Post-treatment:
Growth environment: greenhouse
Light condition: Natural conditions
Temperature: 28±3℃
Growth length: 7 DAI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Separation: trimming
Extraction source: frozen
Extraction source: leaves pooled from 5 individual plants
Kit: TRIzol
Manual: Invitrogen
|
| Label |
Cy3
|
| Label protocol |
Kit: Low RNA Input Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| Channel 2 |
| Source name |
Mock TW16-4, rep 2
|
| Organism |
Oryza sativa Indica Group |
| Characteristics |
Tissue: two youngest leaves
Near isogenic line from Utri Merah and Taiching Native 1
|
| Treatment protocol |
Growth stage: two leaves visible
Light condition: Natural conditions
Temperature: 28±3℃
Virus inoculation: mock inoculation (virus-free GLH)
Virus inoculation: 3 virus-free GLH (Nephotettix virescence) per 1 seedling
GLH inoculation: 24 hours
|
| Growth protocol |
Pre-treatment:
Growth environment: greenhouse
Growth: pot culture (D: 120mm x H: 150mm)
Growth media: Soil collected fielf of International Rice Research Institute, Philippines. Autoclaved prior to use.
No. of seedlings per pot: 5
Light condition: Natural conditions
Temperature: 28±3℃
Post-treatment:
Growth environment: greenhouse
Light condition: Natural conditions
Temperature: 28±3℃
Growth length: 7 DAI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Separation: trimming
Extraction source: frozen
Extraction source: leaves pooled from 5 individual plants
Kit: TRIzol
Manual: Invitrogen
|
| Label |
Cy5
|
| Label protocol |
Kit: Low RNA Input Linear Amplification kit
Manufacturer: Agilent Technologies
Manual: Low RNA Input Linear Amplification kit standard protocol
|
| |
|
| |
| Hybridization protocol |
Hybridization kit: in situ hybridization plus
Hybridization manufacturer: Agilent Technologies
Hybridization manual: Agilent Technologies
|
| Scan protocol |
Scanner: Agilent DNA microarray Scanner
Manufacturer: Agilent Technologies
Software: Scanner Control software
Manual: Agilent Technologies
|
| Description |
Biological replication 2 of 2, RTSV-infected TW16-4 at 7DAI
|
| Data processing |
Software: Feature Extraction version 8.1.1.1
Manufacturer: Agilent Technologies
Manual: Agilent Technologies
|
| |
|
| Submission date |
Jan 27, 2009 |
| Last update date |
Jan 28, 2009 |
| Contact name |
IL-RYONG CHOI |
| E-mail(s) |
ichoi@cgiar.org
|
| Organization name |
International Rice Research Institute
|
| Street address |
DAPO Box 7777
|
| City |
Metro Manila |
| ZIP/Postal code |
0000 |
| Country |
Philippines |
| |
|
| Platform ID |
GPL892 |
| Series (1) |
| GSE14606 |
Transcriptome analysis of rice in relation to infection with rice tungro spherical virus (RTSV) |
|
