strain: Wild-caught cell type: young tissue: Fibroblasts
Treatment protocol
No treatment
Growth protocol
Fibroblasts were maintained at 37 °C incubator in an atmosphere of 5% CO2 and 3% O2 on treated polystyrene culture dishes (Corning) in EMEM media (ATCC) with 15% (vol/vol) fetal bovine serum (FBS).
Extracted molecule
genomic DNA
Extraction protocol
Genomic DNA was extracted using a Zymo Quick-DNA Miniprep Plus Kit. Bisulfite conversion of DNA samples were performed using the Zymo EZ DNA Methylation Kit.
Label
cy3,cy5
Label protocol
Cy3 and Cy5 labeling, hybridization, and scanning (iScan, Illumina), were performed.
Hybridization protocol
Cy3 and Cy5 labeling, hybridization, and scanning (iScan, Illumina), were performed.
Scan protocol
Cy3 and Cy5 labeling, hybridization, and scanning (iScan, Illumina), were performed.
Data processing
DNAm levels (β values) were determined by calculating the ratio of intensities between methylated (signal A) and unmethylated (signal B) sites.
