GEO DataSets

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3137

Platform:

GPL90

6 Samples

Download data: CEL

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Candida albicans; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL6346 GPL90

9 Samples

Download data: CEL, TXT

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL6346

3 Samples

Download data: TXT

(Submitter supplied) The C. albicans Genome Oligo Set consisting of 70mer probes representing 6266 C. albicans genes was obtained from Qiagen (Valencia, CA), and printed on glass slides by Microarrays, Inc (Nashville, TN).

Organism:

Candida albicans

2 Series

3 Samples

Download data

Analysis of Saccharomyces cerevisiae cells treated with the antifungal compound sampangine. Sampangine exhibits strong inhibitory activity against opportunistic fungal pathogens such as Candida albicans. Using S. cerevisiae as a model, results provide insight into sampangine's mechanism of action.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL90

Series:

GSE10073

6 Samples

Download data: CEL

(Submitter supplied) 6-Nonadecynoic acid (6-NDA), a plant-derived acetylenic acid, exhibits strong inhibitory activity against the human fungal pathogens Candida albicans, Aspergillus fumigatus, and Trichophyton mentagrophytes. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Saccharomyces cerevisiae to investigate the mechanism of action of this compound. more...

Organism:

Candida albicans; Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platforms:

GPL15195 GPL2529

14 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) In previous temporal studies, we found the anaerobic response was biphasic when cells growing in galactose medium were shifted from aerobiosis to anaerobiosis, consisting of an acute, transitory phase (<60 min) followed by a more chronic but delayed phase (> 1 generation), but largely monophasic (delayed, chronic phase only) when cells were shifted in glucose medium. Gene network and functional analyses revealed the acute phase was comprised of genes associated with the retooling of metabolism (respiro-fermentative to strictly fermentative) and balancing energy supply and demand. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1535

227 Samples

Download data

(Submitter supplied) In previous temporal studies, we found the anaerobic response was biphasic when cells growing in galactose medium were shifted from aerobiosis to anaerobiosis, consisting of an acute, transitory phase (<60 min) followed by a more chronic but delayed phase (> 1 generation), but largely monophasic (delayed, chronic phase only) when cells were shifted in glucose medium. Gene network and functional analyses revealed the acute phase was comprised of genes associated with the retooling of metabolism (respiro-fermentative to strictly fermentative) and balancing energy supply and demand. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL1535

227 Samples

Download data

(Submitter supplied) Plakortide F acid (PFA), a marine-derived polyketide endoperoxide, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Saccharomyces cerevisiae, to investigate the mechanism of action of this compound. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae; Saccharomyces cerevisiae S288C

Type:

Expression profiling by array

Platform:

GPL2529

6 Samples

Download data: CEL, CHP

(Submitter supplied) Eupolauridine and liriodenine are plant-derived aporphinoid alkaloids that exhibit potent inhibitory activity against the opportunistic fungal pathogens Candida albicans and Cryptococcus neoformans. However, the molecular mechanism of this antifungal activity is unknown. In this study, we show that eupolauridine 9591 (E9591), a synthetic analog of eupolauridine, and liriodenine methiodide (LMT), a methiodide salt of liriodenine, mediate their antifungal activities by disrupting mitochondrial iron-sulfur (Fe-S) cluster synthesis. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

30 Samples

Download data: CEL, CHP

(Submitter supplied) To test the effect of hemin and of HAP1 in C. albicans, a hap1 mutant and it's reintegrant (wild-type) strain were grown in YPD to log phase, and then exposed or not to 50 microM hemin for 30'.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32241

12 Samples

Download data: CSV

(Submitter supplied) Candida albicans was subjected to treatment with tomatidine and samples were taken at 2 different time points (1h, 3h). The results show that tomatidine induces genes involved in ergosterol biosynthesis as compared to untreated controls.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

12 Samples

Download data: XLSX

(Submitter supplied) We used RNA-seq to monitor mRNA levels of all genes in response to hypoxia of wild-type yeast, S. cerevisiae (strain yMH914 with wildtype HAP1). To gain insights into how gene expression changes over time, cells were subjected to 100% nitrogen gas and collected after 0,5,10,30,60,120,180, and 240 minutes. Total RNA was extracted and mRNAs were enriched by polyA selection. The cDNA was prepared into a sequencing library, multiplexed and single-end sequenced by an Illumina HiSeq 2500 sequencer. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17342

24 Samples

Download data: TAB

(Submitter supplied) We addressed changes in gene expression profile in response to reduced oxygen tension in wild type or heme depletion caused by deletion of heme biosynthesis gene hem1 Keywords: Response to heme depletion and hypoxia

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Datasets:

GDS3437 GDS3438

Platform:

GPL90

8 Samples

Download data: CEL

Analysis of Saccharomyces cerevisiae wild type cells grown under reduced oxygen tension. Heme synthesis requires oxygen, and yeast grown anaerobically exhibit an increased uptake of hemin. Results provide insight into the molecular mechanisms regulating heme uptake.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 growth protocol sets

Platform:

GPL90

Series:

GSE9514

4 Samples

Download data: CEL

Analysis of Saccharomyces cerevisiae mutant cells lacking aminolevulinic acid (ALA) synthase. ALA synthase catalyzes the first step of heme biosynthesis. Heme uptake is induced by heme starvation in S. cerevisiae. Results provide insight into the molecular mechanisms regulating heme uptake.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 growth protocol sets

Platform:

GPL90

Series:

GSE9514

4 Samples

Download data: CEL

(Submitter supplied) The cell wall is essential for viability of fungi and is an effective drug target in pathogens such as Candida albicans. The contribution of posttranscriptional gene regulators to cell wall integrity in C. albicans is unknown. We show that the C. albicans Ccr4-Pop2 mRNA deadenylase, a regulator of mRNA stability and translation, is required for cell wall integrity. The ccr4/pop2 mutants display reduced wall β-glucans and sensitivity to the echinocandin caspofungin. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9818

4 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Candida albicans cells comparing control untreated C. albicans cells with sulfite-treated C. albicans cells. Sulfite is a toxic molecule that C. albicans encounters in its human host. Both wild type and ∆zcf2 mutant cells were used. The goal was to determine the effects of sulfite on C. albicans gene expression, and to determine which of the genes areZcf2-depedent.

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL16243

8 Samples

Download data: TXT

(Submitter supplied) Iron is an essential cofactor for enzymes involved in numerous cellular processes. We analyzed the metabolomes and transcriptomes of yeast grown in iron-rich and iron-poor media to determine which biosynthetic processes are altered when iron availability falls.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

8 Samples

Download data: CEL

(Submitter supplied) Growth assay in the presence of a toxic chemical that uses the barcoded collections of yeast gene deletions (haploid, diploid, DamP) to identify deletion strains that are hypersensitive to the drug.

Organism:

Saccharomyces cerevisiae; Saccharomyces cerevisiae S288C

Type:

Expression profiling by array

Platform:

GPL18088

6 Samples

Download data: GPR