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Platform GPL1528 Query DataSets for GPL1528
Status Public on Oct 27, 2004
Title NCI/ATC Hs-OperonV2
Technology type spotted oligonucleotide
Distribution non-commercial
Organism Homo sapiens
Manufacturer Printing by the NCI /ATC Microarray Facility
Manufacture protocol The human long oligonuleotide probe set contains 21,329 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/ ) . The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 micro array) and dissolved in 3XSSC print buffer at a concentration of 20-30 uM. The microarray spot densities are 160 microns (center-to-center), and one spot is printed per probe per slide. The microarrays printed on Corning Ultra-GAPS slides (Item #40018) coated with amino-silane and post-processed according to the manufacturer's protocol.
Support glass
Coating amino-silane coated glass Corning Ultra-GAPS (http://www.corning.com)
 
Description Attachment: UV cross-linked
Array description: The custom arrays are manufactured by the NCI Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC). Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com) that is fitted with a printhead containing 32 SMP3 printing pins from Telechem International (http://www.arrayit.com).

 
Citation(s) 16532004
Submission date Oct 22, 2004
Last update date Jan 17, 2013
Contact name David Petersen
E-mail(s) petersed@mail.nih.gov
Phone 301-435-7898
Organization name NCI
Lab NCI Microarray Facility
Street address 8717 Grovemont Circle
City Gaithersburg
State/province MD
ZIP/Postal code 20892-4605
Country USA
 
Samples (1045) GSM33748, GSM33749, GSM33750, GSM33751, GSM33752, GSM33753 
Series (16)
GSE1898 Human Hepatocellular Carcinoma study
GSE2020 Differential Gene Up-regulation by HIF-1a and HIF-2a in HEK293T.
GSE2296 Effects of inducible HIV-1 Vpr protein on cellular gene expression

Data table header descriptions
ID NIH mAdb well id plus replicate number
MADB_WELL_ID NIH mAdb plate-well identifier for oligo sample
OLIGO_ID Oligo identifier
GENE Gene Name
UNIGENE UniGene Identifier Build #Hs.194
DESCRIPTION Gene Description
GB_LIST Representative GENBANK ID
SPOT_ID spot identifier for features without GB_LIST

Data table
ID MADB_WELL_ID OLIGO_ID GENE UNIGENE DESCRIPTION GB_LIST SPOT_ID
1173588_1 1173588 H200000098 PTS 503860 6-pyruvoyltetrahydropterin synthase (PTS), mRNA. NM_000317
1173592_1 1173592 H200000102 FLT3 507590 fms-related tyrosine kinase 3 (FLT3), mRNA. NM_004119
1173596_1 1173596 H200000106 ADM 441047 adrenomedullin (ADM), mRNA. NM_001124
1173612_1 1173612 H200000122 FUT7 457 fucosyltransferase 7 (alpha (1,3) fucosyltransferase) (FUT7), mRNA. NM_004479
1173616_1 1173616 H200000126 SDHA 440475 succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (SDHA), nuclear gene encoding mitochondrial protein, mRNA. NM_004168
1173620_1 1173620 H200000130 ISL1 505 ISL1 transcription factor, LIM/homeodomain, (islet-1) (ISL1), mRNA. NM_002202
1173636_1 1173636 H200000146 ALDH3A1 531682 aldehyde dehydrogenase 3 family, memberA1 (ALDH3A1), mRNA. NM_000691
1173640_1 1173640 H200000150 GOT1 500756 glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1), mRNA. NM_002079
1173644_1 1173644 H200000154 LGALS3 531081 lectin, galactoside-binding, soluble, 3 (galectin 3) (LGALS3), mRNA. NM_002306
1173660_1 1173660 H200000170 CSTF2 132370 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa (CSTF2), mRNA. NM_001325
1173664_1 1173664 H200000174 CYP2C18 511872 cytochrome P450, family 2, subfamily C, polypeptide 18 (CYP2C18), mRNA. NM_000772
1173668_1 1173668 H200000178 THRA 724 thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) (THRA), transcript variant 2, mRNA. NM_003250
1173972_1 1173972 H200000478 PGC 1867 progastricsin (pepsinogen C) (PGC), mRNA. NM_002630
1173976_1 1173976 H200000482 PRKCH 333907 protein kinase C, eta (PRKCH), mRNA. NM_024064
1173980_1 1173980 H200000486 KLKB1 237642 kallikrein B, plasma (Fletcher factor) 1 (KLKB1), mRNA. NM_000892
1173996_1 1173996 H200000502 SEMG1 1968 semenogelin I (SEMG1), transcript variant 2, mRNA. NM_003007
1174000_1 1174000 H200000506 PDGFB 1976 platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog) (PDGFB), transcript variant 2, mRNA. NM_002608
1174004_1 1174004 H200000510 SRY 1992 sex determining region Y (SRY), mRNA. NM_003140
1174020_1 1174020 H200000526 TYR 503555 tyrosinase (oculocutaneous albinism IA) (TYR), mRNA. NM_000372
1174024_1 1174024 H200000530 ZFX 336681 zinc finger protein, X-linked (ZFX), mRNA. X59739, NM_003410

Total number of rows: 22242

Table truncated, full table size 2315 Kbytes.






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